Bacteroides fragilis metabolises exopolysaccharides produced by bifidobacteria

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Different metabolic features of Bacteroides fragilis growing in the presence of glucose and exopolysaccharides of bifidobacteria

Bacteroides is among the most abundant microorganism inhabiting the human intestine. They are saccharolytic bacteria able to use dietary or host-derived glycans as energy sources. Some Bacteroides fragilis strains contribute to the maturation of the immune system but it is also an opportunistic pathogen. The intestine is the habitat of most Bifidobacterium species, some of whose strains are con...

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Interactions between Bifidobacterium and Bacteroides species in cofermentations are affected by carbon sources, including exopolysaccharides produced by bifidobacteria.

Cocultures of strains from two Bifidobacterium and two Bacteroides species were performed with exopolysaccharides (EPS) previously purified from bifidobacteria, with inulin, or with glucose as the carbon source. Bifidobacterium longum NB667 and Bifidobacterium breve IPLA20004 grew in glucose but showed poor or no growth in complex carbohydrates (inulin, EPS E44, and EPS R1), whereas Bacteroides...

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Diversity of the metalloprotease toxin produced by enterotoxigenic Bacteroides fragilis.

Enterotoxigenic Bacteroides fragilis (ETBF) strains produce a 20-kDa zinc metalloprotease toxin (BFT) associated with diarrheal disease of animals, young children, and adults. BFT stimulates secretion in intestinal loops in vivo and modifies epithelial cell morphology in vitro. The B. fragilis toxin (bft) gene from ETBF strain 86-5443-2-2 (piglet; bft-2) revealed significant nucleotide and pred...

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Active efflux of norfloxacin by Bacteroides fragilis.

Norfloxacin was actively pumped out by Bacteroides fragilis, which is intrinsically resistant to most fluoroquinolones. Reserpine moderately inhibited the efflux. A one-step spontaneous mutant had increased resistance to norfloxacin, ethidium bromide, and puromycin, a result suggesting that the efflux is catalyzed by a multidrug pump with specificity similar to that of NorA/Bmr.

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Accumulation of norfloxacin by Bacteroides fragilis.

The accumulation of norfloxacin by Bacteroides fragilis NCTC 9343 was determined by the modified fluorescence method. The time required to achieve a steady-state concentration (SSC) after allowing B. fragilis to accumulate norfloxacin in an aerobic or an anaerobic environment was approximately 2 min; the SSC achieved in air was 90.28 +/- 9.32 ng of norfloxacin/mg (dry weight) of cells, and that...

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ژورنال

عنوان ژورنال: BMC Microbiology

سال: 2016

ISSN: 1471-2180

DOI: 10.1186/s12866-016-0773-9